This diagnostic kit is used to detect the presence of Herpse Simplex Virus-2 (HSV-2) DNA in samples such as male urethral swab and female cervical swab. The detection result can be used to aid the diagnosis of an HSV-2 infection. The test provides a molecular diagnostics-based solution for early diagnosis of venereal disease and for preliminary screening of high-risk venereal disease groups.
The diagnostic kit adopts a nucleic acid lysis buffer to allow rapid lysis and release of HSV-2 DNA from a reproductive tract secretion sample. By applying real-time fluorescence quantitative PCR technology, this test utilizes a pair of specific primers which are designed to target at a conserved region of HSV-2 DNA, a specific fluorescence probe, accompanied with components such as PCR mix,to achieve quick detection of HSV-2 DNA through the change in fluorescent signals.
The PCR detection system uses UNG enzyme + dUTP contamination-proof system which is able to fully degrade possible unwanted side-products to avoid a false positive result.
The PCR detection system uses an internal control, which monitors the presence of PCR inhibitors in test samples by detecting whether the internal control signal is normal, to avoid a false negative result.