This diagnostic kit is used to detect the presence of Neisseria Gonorrhoeae (NG) -DNA in samples such as male urethral swab and female cervial swab. The detection result can be used to aid the diagnosis of an NG infection. The test provides a molecular diagnostics-based solution for early diagnosis of venereal disease and for preliminary screening of high-risk venereal disease groups.
The diagnostic kit adopts a nucleic acid lysis buffer to allow rapid lysis and release of NG-DNA from a reproductive tract secretion sample. By applying real-time fluorescence quantitative PCR technology, this test utilizes a pair of specific primers which are designed to target at a conserved sequence of NG-DNA, a specific fluorescence probe, accompanied with such components as PCR mix,to achieve quick detection of NG-DNA through the change in fluorescent signals.
The PCR detection system uses UNG enzyme + dUTP contamination-proof system which is able to fully degrade possible unwanted side-products to avoid a false positive result. The PCR detection system uses an internal control to monitor the presence of PCR inhibitors in test samples by detecting whether the internal control signal is normal to avoid a false negative result.