This Chlamydia Trachomatis (CT) DNA Fluorescence Diagnostic Kit is based on real-time PCR method and quantification by amplifying viral DNA directly from clinical male urethral swab and female cervical swab.intended for assisting CT infection diagnosis and The results can provide molecular evidence for early STDs diagnosis also high-risk STD population primary screening .
The diagnostic kit contains a rapid nucleic acid lysis buffer which accelerate viral particles lysis and CT-DNA release from samples. A pair of CT specific primers has been applied in real-time fluorescence quantitative PCR method which targeted at a conserved regions in CT genome with a specific fluorescence probe and PCR mix, CT quantification result can be detected by reading fluorescent signal concomitantly.
A UNG enzyme + dUTP contamination-proof system has been presented in this kit., which reduce contaminations risk by degrading possible unpleasure products through reaction. . An internal control has been used in this PCR detection system , which monitors PCR inhibitors in amplification reaction, this control can avoid false negative result.