Human Cytomegalovirus DNA Quantitative Fluorescence Diagnostic Kit (PCR-Fluorescence Probing)


The diagnostic kit is an in vitro nucleic acid amplification test for the detection of Human Cytomegalovirus (HCMV) DNA in human urine, plasma, human milk or peripheral blood. It is intended for use as an aid in diagnosing an HCMV infection and in observing drug efficacy.

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The diagnostic kit uses a nucleic acid lysis buffer to allow rapid lysis and release of HCMV DNA in test samples. By applying real-time fluorescence quantitative PCR technology, this test utilizes a pair of specific primers which are designed to target at a conserved sequence of HCMV-DNA, a specific fluorescence probe, accompanied with PCR mix, to achieve fast detection of HCMV-DNA through fluorescent signal changes.

The PCR detection system uses UNG enzyme + dUTP contamination-proof system, which can fully degrade possible unwanted side-products, to avoid a false positive result.

The PCR detection system uses internal control to judge whether the internal control is normal by monitoring the presence of PCR inhibitors, so as to avoid a false negative result.

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