This diagnostic kit is an in vitro nucleic acid amplification test for the detection of mycoplasma pneumonia (MP) DNA in human sputum and throat swab. It is intended for use as an aid in diagnosing MP infection and providing molecular-diagnostics-based solution.
The diagnostic kit uses a nucleic acid lysis buffer to allow rapid lysis and release of MP-DNA from a sputum specimen or throat swab which has been processed with concentrated. By applying real-time fluorescence PCR technology, this test utilizes a pair of specific primers which are designed to target at a conserved sequence of MP-DNA, a specific fluorescence probe, accompanied with PCR mix, to achieve quick detection of MP-DNA through fluorescent signal changes.
The PCR detection system uses UNG enzyme + dUTP contamination-proof system, which can fully degrade possible unwanted side-products, to avoid a false positive result.
The PCR detection system uses internal control to monitor the presence of PCR inhibitors in order to avoid a false negative result.